This page provides a structured overview of the main topics in cell culture.
First, fundamental concepts are introduced. Then, laboratory setup and aseptic techniques are described.
Finally, practical protocols and methods for cell culture analysis are presented.
1. Introduction
- advantages of cell culture 1: Environmental control
- advantages of cell culture 2: Cellular homogeneity
- advatanges of cell culture 3: other factors
- Limitation of cell cultures 1: Phenotypic and genotypic variability
- Limitations of cell culture 2: morphological and functional differences with cells in vivo
2. layout of the cell culture laboratory and necessary equipment
- laminar-flow hood
- Laminar flow hood: selection and installation.
- Laminar flow hood: working tips
- Water bath: mainteinance and usage protocol to avoid contamination
3. Aseptic technique
- Decontamination
- aseptic technique SOP
- A guide to cell-lab personal hygiene
- Other tips for sterile handling
4. Culture media
4.1 Phisicochemical properties
4.2 The components of cell culture medium
- The components of the cell culture medium: HEPES
- The components of the cell culture medium: glutamine
- Phenol red
5. Culture Vessels
6. Media and reagents
- Media, reagents, and solution preparation
- Preparation of cell culture media
- PBS recipe and preparation
- Protocol: 4% Paraformaldehyde solution in PBS
- Scott’s tap water substitute
7. Subculture
8. Quantitation
- how to calculate the cell seeding density
- In how much volume resuspend the cells to plate them at the desired cell density?
- Cell counting
- Methods to estimate Cell Confluency
9. Contamination
- Cell culture contamination: main causes
- Contaminated! When you see it by eye
- Contamination: when it is hard to detect – Mycoplasma
- Summary of contamination preventive methods
- Summary of Contamination Containment Methods
- Decontamination
- Eradication of fungus and mold contamination
- Eradication of bacteria contamination
- Eradication of mycoplasma contamination: antibiotics